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liquid scintillation vials  (Thermo Fisher)


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    Structured Review

    Thermo Fisher liquid scintillation vials
    Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
    Liquid Scintillation Vials, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/liquid+scintillation+vials/liquid+scintillation+vials/pmc12274924-21-0-4
    Average 90 stars, based on 1 article reviews
    liquid scintillation vials - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells"

    Article Title: Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells

    Journal: STAR Protocols

    doi: 10.1016/j.xpro.2025.103935

    Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.
    Figure Legend Snippet: Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.

    Techniques Used: In Vitro, Binding Assay

    Related Articles

    In Vitro:

    Article Title: Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells
    Article Snippet: Avanti J-E centrifuge , Beckman Coulter , N/A. .. Liquid scintillation vials , Thermo Fisher Scientific , Cat#S31. .. Bio-Rad Poly-Prep chromatography columns , Bio-Rad , C

    Binding Assay:

    Article Title: Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells
    Article Snippet: Avanti J-E centrifuge , Beckman Coulter , N/A. .. Liquid scintillation vials , Thermo Fisher Scientific , Cat#S31. .. Bio-Rad Poly-Prep chromatography columns , Bio-Rad , C



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    Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid <t>scintillation</t> counter.
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    Image Search Results


    Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.

    Journal: STAR Protocols

    Article Title: Protocol to measure endogenous GlcNAc-1-phosphotransferase activity in SK-MEL-30 cells

    doi: 10.1016/j.xpro.2025.103935

    Figure Lengend Snippet: Setting up the in vitro GNPT assay (A) Drying down of the [ 3 H]UDP-GlcNAc donor molecule mixed with cold UDP-GlcNAc and ATP using a Speed Vac Concentrator attached to a cold trap that is connected to a vacuum pump. (B) Sonicator with attached microprobe used to prepare whole cell lysate. (C) Equilibration of QAE Sephadex column. (D) Binding of the reaction mixture to the QAE Sephadex column and washing of the column. (E and F) First and second elutions with 2 mM Tris buffer containing 30 mM NaCl. (G) Detecting counts in the elutions using a liquid scintillation counter.

    Article Snippet: Liquid scintillation vials , Thermo Fisher Scientific , Cat#S31.

    Techniques: In Vitro, Binding Assay